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Fig. 6. S100A8 knockdown inhibits excessive autophagy in the hippocampus of CSD mice A: Autophagosomes in the hippocampus examined by transmission electron microscopy microscopy. The autophagosomes are denoted by arrows. Scale bar: 1 µm. B-E: An Immunohistochemical staining assay was performed to detect the LC3B, P62 and Beclin-1 levels in hippocampal tissues. Scale bars: 200 and 20 µm F: Detection of S100A8 and apoptosis-related proteins in the hippocampal tissues of mice in each group by Western blot analysis. G: Western blot analysis of the protein expression of phosphorylated PI3K, Akt and <t>P70S6K</t> in hippocampal tissue H- N: Analysis of the protein band gray intensity. n = 5–6 mice per group; *P < 0.05 vs Control; #P < 0.05 vs CSD; &P < 0.05 vs Si-NC + CSD; $P < 0.05 vs Si-S100A8 + CSD.
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Fig. 6. S100A8 knockdown inhibits excessive autophagy in the hippocampus of CSD mice A: Autophagosomes in the hippocampus examined by transmission electron microscopy microscopy. The autophagosomes are denoted by arrows. Scale bar: 1 µm. B-E: An Immunohistochemical staining assay was performed to detect the LC3B, P62 and Beclin-1 levels in hippocampal tissues. Scale bars: 200 and 20 µm F: Detection of S100A8 and apoptosis-related proteins in the hippocampal tissues of mice in each group by Western blot analysis. G: Western blot analysis of the protein expression of phosphorylated PI3K, Akt and <t>P70S6K</t> in hippocampal tissue H- N: Analysis of the protein band gray intensity. n = 5–6 mice per group; *P < 0.05 vs Control; #P < 0.05 vs CSD; &P < 0.05 vs Si-NC + CSD; $P < 0.05 vs Si-S100A8 + CSD.
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Fig. 6. S100A8 knockdown inhibits excessive autophagy in the hippocampus of CSD mice A: Autophagosomes in the hippocampus examined by transmission electron microscopy microscopy. The autophagosomes are denoted by arrows. Scale bar: 1 µm. B-E: An Immunohistochemical staining assay was performed to detect the LC3B, P62 and Beclin-1 levels in hippocampal tissues. Scale bars: 200 and 20 µm F: Detection of S100A8 and apoptosis-related proteins in the hippocampal tissues of mice in each group by Western blot analysis. G: Western blot analysis of the protein expression of phosphorylated PI3K, Akt and <t>P70S6K</t> in hippocampal tissue H- N: Analysis of the protein band gray intensity. n = 5–6 mice per group; *P < 0.05 vs Control; #P < 0.05 vs CSD; &P < 0.05 vs Si-NC + CSD; $P < 0.05 vs Si-S100A8 + CSD.
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Fig. 6. S100A8 knockdown inhibits excessive autophagy in the hippocampus of CSD mice A: Autophagosomes in the hippocampus examined by transmission electron microscopy microscopy. The autophagosomes are denoted by arrows. Scale bar: 1 µm. B-E: An Immunohistochemical staining assay was performed to detect the LC3B, P62 and Beclin-1 levels in hippocampal tissues. Scale bars: 200 and 20 µm F: Detection of S100A8 and apoptosis-related proteins in the hippocampal tissues of mice in each group by Western blot analysis. G: Western blot analysis of the protein expression of phosphorylated PI3K, Akt and <t>P70S6K</t> in hippocampal tissue H- N: Analysis of the protein band gray intensity. n = 5–6 mice per group; *P < 0.05 vs Control; #P < 0.05 vs CSD; &P < 0.05 vs Si-NC + CSD; $P < 0.05 vs Si-S100A8 + CSD.
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Fig. 6. S100A8 knockdown inhibits excessive autophagy in the hippocampus of CSD mice A: Autophagosomes in the hippocampus examined by transmission electron microscopy microscopy. The autophagosomes are denoted by arrows. Scale bar: 1 µm. B-E: An Immunohistochemical staining assay was performed to detect the LC3B, P62 and Beclin-1 levels in hippocampal tissues. Scale bars: 200 and 20 µm F: Detection of S100A8 and apoptosis-related proteins in the hippocampal tissues of mice in each group by Western blot analysis. G: Western blot analysis of the protein expression of phosphorylated PI3K, Akt and <t>P70S6K</t> in hippocampal tissue H- N: Analysis of the protein band gray intensity. n = 5–6 mice per group; *P < 0.05 vs Control; #P < 0.05 vs CSD; &P < 0.05 vs Si-NC + CSD; $P < 0.05 vs Si-S100A8 + CSD.
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Fig. 6. S100A8 knockdown inhibits excessive autophagy in the hippocampus of CSD mice A: Autophagosomes in the hippocampus examined by transmission electron microscopy microscopy. The autophagosomes are denoted by arrows. Scale bar: 1 µm. B-E: An Immunohistochemical staining assay was performed to detect the LC3B, P62 and Beclin-1 levels in hippocampal tissues. Scale bars: 200 and 20 µm F: Detection of S100A8 and apoptosis-related proteins in the hippocampal tissues of mice in each group by Western blot analysis. G: Western blot analysis of the protein expression of phosphorylated PI3K, Akt and <t>P70S6K</t> in hippocampal tissue H- N: Analysis of the protein band gray intensity. n = 5–6 mice per group; *P < 0.05 vs Control; #P < 0.05 vs CSD; &P < 0.05 vs Si-NC + CSD; $P < 0.05 vs Si-S100A8 + CSD.
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Fig. 6. S100A8 knockdown inhibits excessive autophagy in the hippocampus of CSD mice A: Autophagosomes in the hippocampus examined by transmission electron microscopy microscopy. The autophagosomes are denoted by arrows. Scale bar: 1 µm. B-E: An Immunohistochemical staining assay was performed to detect the LC3B, P62 and Beclin-1 levels in hippocampal tissues. Scale bars: 200 and 20 µm F: Detection of S100A8 and apoptosis-related proteins in the hippocampal tissues of mice in each group by Western blot analysis. G: Western blot analysis of the protein expression of phosphorylated PI3K, Akt and <t>P70S6K</t> in hippocampal tissue H- N: Analysis of the protein band gray intensity. n = 5–6 mice per group; *P < 0.05 vs Control; #P < 0.05 vs CSD; &P < 0.05 vs Si-NC + CSD; $P < 0.05 vs Si-S100A8 + CSD.
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Image Search Results


Fig. 6. S100A8 knockdown inhibits excessive autophagy in the hippocampus of CSD mice A: Autophagosomes in the hippocampus examined by transmission electron microscopy microscopy. The autophagosomes are denoted by arrows. Scale bar: 1 µm. B-E: An Immunohistochemical staining assay was performed to detect the LC3B, P62 and Beclin-1 levels in hippocampal tissues. Scale bars: 200 and 20 µm F: Detection of S100A8 and apoptosis-related proteins in the hippocampal tissues of mice in each group by Western blot analysis. G: Western blot analysis of the protein expression of phosphorylated PI3K, Akt and P70S6K in hippocampal tissue H- N: Analysis of the protein band gray intensity. n = 5–6 mice per group; *P < 0.05 vs Control; #P < 0.05 vs CSD; &P < 0.05 vs Si-NC + CSD; $P < 0.05 vs Si-S100A8 + CSD.

Journal: International immunopharmacology

Article Title: S100A8 knockdown activates the PI3K/AKT signaling pathway to inhibit microglial autophagy and improve cognitive impairment mediated by chronic sleep deprivation.

doi: 10.1016/j.intimp.2024.113375

Figure Lengend Snippet: Fig. 6. S100A8 knockdown inhibits excessive autophagy in the hippocampus of CSD mice A: Autophagosomes in the hippocampus examined by transmission electron microscopy microscopy. The autophagosomes are denoted by arrows. Scale bar: 1 µm. B-E: An Immunohistochemical staining assay was performed to detect the LC3B, P62 and Beclin-1 levels in hippocampal tissues. Scale bars: 200 and 20 µm F: Detection of S100A8 and apoptosis-related proteins in the hippocampal tissues of mice in each group by Western blot analysis. G: Western blot analysis of the protein expression of phosphorylated PI3K, Akt and P70S6K in hippocampal tissue H- N: Analysis of the protein band gray intensity. n = 5–6 mice per group; *P < 0.05 vs Control; #P < 0.05 vs CSD; &P < 0.05 vs Si-NC + CSD; $P < 0.05 vs Si-S100A8 + CSD.

Article Snippet: Antibodies against GFAP (#3670), LC3B (#83506), phosphophosphoinositide 3-kinase (PI3K) p85 (#4228), phospho-Akt (#13038), PI3K p85 (#4292), Akt (#4685), phospho-P70S6K (#97596), and P70S6K (#9202) were obtained from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Knockdown, Transmission Assay, Electron Microscopy, Microscopy, Immunohistochemical staining, Staining, Western Blot, Expressing, Control